Getting My cytometers To Work
Getting My cytometers To Work
Blog Article
Details-enriched characterization of cell populations. Morphological information and facts from photographs can add into the richness of flow cytometry details. As an example, the figure reveals an or else traditional apoptosis assay working with Annexin V and PI, incorporating mobile imaging to characterize cells in Each and every population to reveal morphologically distinctive options. These insights could not have already been gained from multiplex staining by itself.
Whether or not you’re a newbie or a specialist, explore our assets to progress your study and knowledge in flow cytometry.
(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with respect to particle rely due to their complexity and noncircular borders. The volume of singlets accessible for Examination improves when the user trains and employs a more exact product.
Uncover new insights into your experimental info by visualizing populations working with mixed fluorescence and morphology facts. Our program interface wizards enable downsampling, concatenation (merged data files) and dimensionality instruments which are created in to the software package for an intuitive, streamlined data Investigation expertise.
"A picture is not rather value a thousand dots, but it genuinely helps you to see photographs. It is great to view photographs that correlate with staining. Such as, AnnexinV-constructive cells that happen to be morphologically unique than nutritious cells, or CD14+ monocytes that happen to be more substantial plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a qualified consumer has gated singlets confidently. Soon after evaluating the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of more than 4% aggregates.
Pair your Attune flow cytometer by having an autosampler for amplified workflow performance, and increase robotic integration for maximum throughput.
Our scientists will design panels in your case as again and again you would like—cost-free, no product or service acquire essential.
Early indications of this contamination experienced originally been dismissed as particles. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also allows you to use morphological functions to find interesting subpopulations that will not be obvious from flow cytometry knowledge alone.
Maybe most importantly, these gatherings contain cells of Evidently distinct phenotypes which could lead to incorrect conclusions pertaining to double constructive occasions (especially in scarce populations).
AI-pushed automatic graphic analysis While using the Attune Cytometric Computer software interprets celebration features into distinct morphology parameters that can be coupled with conventional fluorescence and scatter parameters to expose extremely reproducible inhabitants modifications and unique subsets.
Software Notice: Automated picture Investigation minimizes consumer-to-user variability in flow cytometry gating tactics
Leverage the power of advanced engineering and innovation to accelerate your discoveries. Have confidence in in the Attune Flow Cytometer spouse and children to provide the reliability, pace, and precision that established your work apart.
Versatile payment strategy from Agilent Fiscal Options help you obtain the crucial instrumentation you require for speedy undertaking, or to develop your lab.
At the guts of every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which utilizes audio waves to meticulously align cells within the flow check here cell. This revolutionary strategy surpasses traditional hydrodynamic techniques, causing more quickly processing speeds, Extraordinary info top quality, and increased sample throughput.
The Attune Xenith cytometer is a high-parameter spectral analyzer, giving both of those spectral unmixing and conventional flow abilities for extensive and adaptable cell Investigation.
Agilent Benefit Guarantee ensures you at least 10 years of instrument use with the day you buy. In any other case, We are going to credit history you While using the residual worth of the system towards an upgraded design.
What appeared to be a homogeneous inhabitants is actually additional diverse—an Perception that needs to be regarded as when interpreting final results.